Tervetuloa kirjastoon!

*00002832cam a22003614i 4500
*00110842
*00520210906140754.0
*008210906s2021    xxup     b    001 0 eng c
*020  $a978-1-119-64078-3$c59,68$qnidottu
*035  $a(FI-MELINDA)018028628
*040  $bmul$erda$dFI-T
*0410 $aeng
*05000$aQH442.2
*06000$aQU 550.5.C5
*08200$a572.8/633$223
*084  $aX
*1001 $aBrown, T. A.$q(Terence A.),$ekirjoittaja.
*24510$aGene cloning and DNA analysis :$ban introduction /$cT. A. Brown.
*250  $aEighth edition.
*260  $aOxford$bWiley Blackwell,$c2021
*264 1$aHoboken, NJ :$bWiley-Blackwell,$c2021.
*300  $axv, 416 sivua :$bkuvitettu ;$c25 cm
*336  $ateksti$btxt$2rdacontent
*337  $akäytettävissä ilman laitetta$bn$2rdamedia
*338  $anide$bnc$2rdacarrier
*500  $aMOLE-213A Geenitekniikka
*500  $aPrevious edition: Seventh edition, 2016. biotiet.tdk
*504  $aIncludes bibliographical references and index.
*5050 $aWhy gene cloning and DNA analysis are important -- Vectors for gene cloning : plasmids and bacteriophages -- Purification of DNA from living cells -- Manipulation of purified DNA -- Introduction of DNA into living cells -- Cloning vectors for Escherichia coli -- Cloning vectors for eukaryotes -- How to obtain a clone of a specific gene -- The polymerase chain reaction -- Sequencing genes and genomes -- Studying gene expression and function -- Studying genomes -- Studying transcriptomes and proteomes -- Production of protein from cloned genes -- Gene cloning and DNA analysis in medicine -- Gene cloning and DNA analysis in agriculture -- Gene cloning and DNA analysis in forensic science and archaeology.
*520  $a"The main development in DNA technology since publication of the Seventh Edition of Gene Cloning has been the increased use of gene editing as a tool in both research and biotechnology. The basic methodology for CRISPR editing is now described in Chapter 12 and the applications of the method are explored, in the context of plant genetic engineering, in Chapter 16. Elsewhere, the continuing evolution of next-generation DNA sequencing is reflected by a reorganization of this part of Chapter 10, and to deal with the further proliferation of methods for studying transcriptomes and proteomes (albeit not strictly DNA Analysis) I have created a new chapter devoted to these methods. Other additions include new sections on melt curve analysis of real-time PCR products and genetic typing of human disease mutations"--$cProvided by publisher.
*65012$aCloning, Molecular
*65022$aSequence Analysis, DNA
*65022$aDNA, Recombinant$xanalysis
*77608$iVerkkoaineisto:$z9781119640677
*77608$iOnline version:$aBrown, T. A. (Terence A.),$tGene cloning and DNA analysis$bEighth edition.$dHoboken, NJ : Wiley-Blackwell, 2021.$z9781119640752$wIMP(FI-MELINDA)(DLC) 2020029237
^

Known worldwide as the standard introductory text to this important and exciting area of study, Gene Cloning and DNA Analysis: An Introduction, 8th Edition preserves the tradition of excellence created by previous editions. Comprehensive and authoritative, the book explores all of the topics crucial to an understanding of gene cloning in an approachable way. An easy-to-follow and user-friendly layout is presented in full-color throughout the volume, making it simple to absorb the clear and accessible material contained within. Gene Cloning and DNA Analysis: An Introduction, 8th Edition contains updated and extended coverage of gene editing strategies like CRISPR/Cas, rewritten chapters on DNA sequencing and genome studies, as well as new material on real-time PCR and typing of human disease mutations. Over 250 full-color illustrations are included to bring to life the comprehensive content. The book also covers topics like: The strategies used by researchers and industry practitioners to assemble genome sequences Next generation sequencing methods and descriptions of their applications in studying genomes and transcriptomes Includes the use and application of gene editing strategies Interbreeding between Neanderthals and Homo Sapiens Gene Cloning and DNA Analysis: An Introduction, 8th Edition is an invaluable introductory text for students in classes like genetics and genomics, molecular biology, biochemistry, immunology, and applied biology. It also belongs on the bookshelves of every professional who desires to improve their understanding of the basics of gene cloning or DNA analysis.